Beijing Children's Hospital 1 article published in JoVE Chemistry Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach Lesheng Zhang*1, Tom Cambron*2, Yueqing Niu1, Zigang Xu3, Ning Su4, Hongyan Zheng4, Karl Wei2, Paula Ray2 1Procter and Gamble, Beijing Innovative center, 2Procter and Gamble, Mason Business Center, 3 Here, we present a protocol for collection of confocal Raman spectra from human subjects in clinical studies combined with chemometric approaches for spectral outlier removal and the subsequent extraction of key features.