Hospital of Chengdu University of Traditional Chinese Medicine 12 articles published in JoVE Medicine Immunofluorescent Labeling in Nasal Mucosa Tissue Sections of Allergic Rhinitis Rats via Multicolor Immunoassay Yucheng Hu*1, Hongping Luo*1, Junjie Li1, Rong Zhang1, Linyou Fu1, Qiuyi Ren1, Yue Chen1, Xueting Huang1, Zhihang Zhou1, Hao Yuan1, Li Tian1, Xiaobo Wang2 1Hospital of Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine This protocol describes a multicolor immunofluorescence technique for evaluating the rat model of allergic rhinitis. Medicine Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model Junjie Li1, Yuchen Hu1, Haoming You1, Ruxu Li1, Qiang Ran1, Tianxin Ouyang1, Yong Huang2 1Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese Medicine This protocol presents an economical and efficient method for quantitative evaluation of bone microarchitecture in a mouse model of osteoporosis by combining Hematoxylin-Eosin (HE) staining and micro-computed tomography (Micro-CT) techniques. Medicine A Preliminary Study on Warm Acupuncture and Moxibustion for Treating Chronic Obstructive Pulmonary Disease with Abdominal Distension Shishuang Yu*1, Tianbao Wang*2, Rongtao Ying1, Yi Zhu1, Ke Liu1, Fanyi Yuan1, Hongjing Yang1, Keling Chen1, Jing Shi3, Xialing Luo1 1Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of General Diseases, Sichuan Cancer Hospital, 3Department of Nursing, Hospital of Chengdu University of Traditional Chinese Medicine Here, we present a protocol to properly manipulate warm acupuncture and moxibustion for treating chronic obstructive pulmonary disease (COPD) with abdominal distension. Medicine Acupoint Application Combined with Acupoint Massage for Treating Constipation in a Patient with Chronic Obstructive Pulmonary Disease Fanyi Yuan*1, Feifei Lu*1, Yaoyao Guo*1, Chuantao Zhang1 1Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine Here we demonstrate acupoint application combined with acupoint massage for treating constipation in a patient with chronic obstructive pulmonary disease (COPD). Medicine Auricular Acupuncture as a Traditional Chinese Medicine Therapy for Chronic Obstructive Pulmonary Disease Combined with Sleep Disorders Yijia Xia*1, Tianbao Wang*2, Zhipeng Ma1, Rongtao Ying1, Shiliang Shao1, Jurong Zeng1, Chuantao Zhang1 1Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2General Department, Sichuan Cancer Hospital This protocol introduces an effective method to improve the sleep condition of patients with chronic obstructive pulmonary disease and alleviate cough symptoms by using auricular acupuncture techniques to percutaneously stimulate specific acupoints in the patients' ears. Medicine Ear Plaster Therapy as a Safe and Effective Treatment for Gestational Vomiting Shiliang Shao1, Wenjuan Zheng1, Yi Wen1 1Hospital of Chengdu University of Traditional Chinese Medicine The presented protocol describes a traditional Chinese medicine treatment procedure known for its safety and effectiveness in managing gestational vomiting. Medicine Effects of Mindfulness Training Combined with Tai Chi in Patients with Diabetic Peripheral Neuropathy Yan Yue*1, Min Deng*1, Shunqi Liao1, Guojin Xiao1, Yan Huang1 1Hospital of Chengdu University of Traditional Chinese Medicine When studying exercise for treating diabetic peripheral neuropathy (DPN), achieving mind-body exercise is essential. This study proposes a protocol for mindfulness training combined with Tai Chi to achieve mind-body exercise. Medicine Acupoint Needle-Embedding Combined with Ironing Therapy for Postoperative Pain After Anal Surgery Xiao Tang1,2, Jian Kang1, Ya Liu1 1Hospital of Chengdu University of Traditional Chinese Medicine, 2Chengdu University of Traditional Chinese Medicine The high incidence of pain after anal surgery is due to the physiological structure and pathological status of the anus. At present, the use of painkillers cannot provide satisfactory pain relief, which makes complementary therapy important. This paper proposes acupoint needle-embedding combined with ironing therapy to relieve postoperative pain. Medicine A Traditional Chinese Medicine Characteristic Therapy for Bronchial Asthma: Moxibustion Weiting Sun*1, Ke Liu*2, Ren Yang*3, Liuxue Guo1, Peiyang Gao3 1Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Department of Respiratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 3Hospital of Chengdu University of Traditional Chinese Medicine This protocol presents a curative procedure of moxibustion in treating patients with bronchial asthma. Medicine Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods Haozhou Huang*1,2, Mengqi Li*3, Chuanhong Luo4, Sanhu Fan5, Taigang Mo5, Li Han4, Dingkun Zhang4, Junzhi Lin6 1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine, 3Sichuan Nursing Vocational College, 4State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 5Sanajon Pharmaceutical Group, 6TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine Here, we introduce a stability evaluation method based on multiple light scattering technology to evaluate the stability of traditional Chinese medicine extracts. Chemistry An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry Hui Ye1, Xuemei Liu1, JiaBao Liao2, Haozhou Huang3, Lin Huang1, Yang Bao1, Hongyan Ma1, Junzhi Lin4, Xiaoming Bao5, Dingkun Zhang1, Runchun Xu1 1State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy School, Chengdu University of Traditional Chinese Medicine, 2China Resources Sanjiu Modern Chinese Medicine Pharmaceutical Co., Ltd, 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, 5Shimadzu (China) Co., Ltd Here, a headspace gas chromatography-tandem quadrupole mass spectrometry (HS-GC-MS/MS) method suitable for the determination of trimethylamine (TMA) in animal-derived medicines is described. The protocol includes sample pretreatment, headspace treatment, analysis conditions, methodological validation, and the determination of TMA in animal-derived medicines. Medicine Enema of Traditional Chinese Medicine for Patients with Severe Acute Pancreatitis Shi Wang1,2, Tong Zhou1,2, Kunlan Long1, Jun Chen1, Xiujuan Zhou1, Peiyang Gao1 1Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Clinical Medicine Department, Chengdu University of Traditional Chinese Medicine An enema is a type of traditional Chinese medicine (TCM) therapy used to treat severe acute pancreatitis (SAP). The therapy involves instilling a TCM liquid into the rectum and colon through the anus to keep the liquid in the intestine, absorbing through the intestinal mucosa. This study aims to standardize the enema procedure for SAP patients.