Fujian Medical University 5 articles published in JoVE Biology Epon Post Embedding Correlative Light and Electron Microscopy Shuyuan Wang*1, Haiyan Xiong*1,2, Qiyuan Chang*1,3, Xudong Zhuang*4,5,6, Yaochen Wu1,3, Xinrui Wang4,5,6, Congxian Wu1, Zhifei Fu1,7,8 1Public Technology Service Center, Fujian Medical University, 2The School of Pharmacy, Fujian Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 5Medical Research Center, Fujian Maternity and Child Health Hospital, 6NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, 7Institute of Neuroscience, Fujian Medical University, 8Fujian Key Laboratory of Molecular Neurology, Fujian Medical University We present a detailed protocol for Epon post-embedding correlative light and electron microscopy using a fluorescent protein called mScarlet. This method can maintain the fluorescence and the ultrastructure simultaneously. This technique is amenable to a wide variety of biological applications. Biology Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System Meng-Fei Xu1,2, Jie Chen1,2, Yue Xu1,2, Jing-Lian Zhang1,2, Yi Zhou1,2, Jie-Jie He1,2, Shan Wu1,2, Ya-Lan Wei3,4, Zhen-Yu She1,2 1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 4Medical Research Center, Fujian Maternity and Child Health Hospital This article reports the construction of centromere-associated protein-E (CENP-E) knockout cells using the CRISPR/Cas9 system and three phenotype-based screening strategies. We have utilized the CENP-E knockout cell line to establish a novel approach to validate the specificity and toxicity of the CENP-E inhibitors, which is useful for drug development and biological research. Medicine Plasma Polishing as a New Polishing Option to Reduce the Surface Roughness of Porous Titanium Alloy for 3D Printing Zhiwei Lin*1,2, Lincong Luo*3, Dongxin Lin*4, Yuping Deng4,5, Yang Yang4, Xuecheng Huang6, Tingrui Wu1, Wenhua Huang1,2,4 1Orthopedic Center, Affiliated Hospital of Guangdong Medical University, 2The School of Basic Medical Sciences, Guangdong Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, 5Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, 6Shenzhen Hospital of Guangzhou University of Chinese Medicine (Futian) Plasma polishing is a promising surface processing technology, especially suitable for 3D printing of porous titanium alloy workpieces. It can remove semi-molten powders and ablative oxide layers, thereby effectively reducing surface roughness and improving surface quality. Behavior Usability Evaluation of Augmented Reality: A Neuro-Information-Systems Study Jun Wu*1, Di Zhang*1, Tao Liu2,3,4, Helen Hong Yang5, Yi Wang1, Huili Yao1, Shinan Zhao1 1Economics and Management School, Jiangsu University of Science and Technology, 2School of Health, Fujian Medical University, 3School of Management, Shanghai University, 4School of Management, Zhejiang University, 5Business School, La Trobe University This study presents an experimental paradigm for a usability test combining subjective and objective evaluations. The objective evaluation adopted Neuro-Information-Systems (NeuroIS) methods, and the subjective evaluation adopted a usability questionnaire and a NASA-Task Load Index (NASA-TLX) scale. Biology Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry Meng-Fei Xu1,2, Yu-Hao Yang1,2, Ya-Lan Wei3,4, Jing-Lian Zhang1,2, Xuan Lin1,2, Xiao-Yan Lin1,2, Hui Chen1,2, Zhen-Yu She1,2 1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University This article reports an in vivo inhibition of CENP-E through abdominal surgery and testicular injection of GSK923295, a valuable model for male meiotic division. Using the immunofluorescence, flow cytometry and transmission electron microscopy assays, we show that CENP-E inhibition results in chromosome misalignment and genome instability in mouse spermatocytes.
