The Sixth Affiliated Hospital of Guangzhou Medical University 1 article published in JoVE Medicine Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat Limei Wang1, Jianmin Guo1, Xiaoman Zhong1, Jian Zhang2, Yali Sheng1, Qiwen Lai1, Hui Song1, Wei Yang1 1Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, TCM Non-clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Engineering Research Center for Innovative Drug Evaluation and Research, 2Department of Biomedical Engineering, School of Basic Medical Sciences, the Sixth Affiliated Hospital of Guangzhou Medical University The present protocol describes a simple and efficient method for collecting blood from the subclavian vein in rats. It enables rapid, timely, and easily identifiable sampling without anesthesia and obtains high-quality blood through repetitive sample collection.