Xi'an Medical University 2 articles published in JoVE Bioengineering Fabrication of Decellularized Spleen Matrix Derived from Rats Lifei Yang1, Yerong Qian2, Aihua Shi1, Shasha Wei1, Xin Liu4, Yi Lv1,2,3, Junxi Xiang2, Peng Liu3 1Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 3Department of Graduate School, Xi'an Medical University The decellularized spleen matrix (DSM) holds promising applications in the field of liver tissue engineering. This protocol outlines the procedure for preparing rat DSM, which includes harvesting rat spleens, decellularizing them through perfusion, and evaluating the resulting DSM to confirm its characteristics. Developmental Biology A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo Wei Zhang*1, Xinjuan Lei*1, Xin Zhou*2,3, Boling He1, Liqin Xiao1, Huimin Yue1, Shulin Wang1, Yuting Sun1, Yajun Wu1, Liyang Wang1,4, George Ghartey-Kwansah1, Odell D. Jones5, Joseph L. Bryant6, MengMeng Xu7, Jianjie Ma3, Xuehon Xu1 1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2 Here, we describe a protocol for detection and localization of Drosophila embryo protein and RNA from collection to pre-embedding and embedding, immunostaining, and mRNA in situ hybridization.
