Qiqihar University 1 article published in JoVE Biology An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates Huiwen Jiao1,2, Cheng Lyu3, Weihui Xu1,2, Wenjing Chen1,2, Yunlong Hu1,2, Zhigang Wang1,2 1School of Life Science and Agriculture Forestry, Qiqihar University, 2Heilongjiang Provincial Technology Innovation Center of Agromicrobial Preparation Industrialization, 3School of Foreign Languages, Qiqihar University Here, we present an improved chemotaxis assay protocol. The goal of this protocol is to reduce the steps and costs of traditional bacterial chemotaxis methods and to serve as a valuable resource for understanding plant-microbe interactions.