Felsenstein Medical Research Center 1 article published in JoVE Cancer Research Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs Neta Moskovits1,2, Ella Itzhaki1,2, Nataly Tarasenko1,2, Eva Chausky1,2, Avital Bareket-Samish3, Aleksandr Kaufman1,2, Raisa Meerson1,2, Salomon M. Stemmer1,2,4 1Felsenstein Medical Research Center, 2Davidoff Center, Rabin Medical Center, 3BioInsight Ltd., 4Sackler Faculty of Medicine, Tel Aviv University The present protocol describes generating 3D tumor culture models from primary cancer cells and evaluating their sensitivity to drugs using cell-viability assays and microscopic examinations.