Indian Institute of Technology Jodhpur 1 article published in JoVE Neuroscience Obtaining Human Microglia from Adult Human Brain Tissue Ishan Agrawal1, Shivanjali Saxena1, Preethika Nair1, Deepak Jha2, Sushmita Jha1 1Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, 2Department of Neurosurgery, All India Institute of Medical Sciences Jodhpur This protocol is an efficient, cost effective and robust method of isolating primary microglia from live, adult, human brain tissue. Isolated primary human microglia can serve as a tool for studying cellular processes in homeostasis and disease.