Jeonbuk National University 1 article published in JoVE Biology Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests Yao-Chia Liu1, Nian-Tong Ni1, Ju-Chun Chang1, Yi-Hsuan Li1, Mi Rong Lee2, Jae Su Kim2,3, Yu-Shin Nai1 1Department of Entomology, National Chung Hsing University, 2Department of Agricultural biology, Jeonbuk National University, 3Department of Agricultural Convergence Technology, Jeonbuk National University Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.