Bangabandhu Sheikh Mujibur Rahman Agricultural University 1 article published in JoVE Environment A Novel Method for the Pentosan Analysis Present in Jute Biomass and Its Conversion into Sugar Monomers Using Acidic Ionic Liquid Babasaheb M. Matsagar1, Shahriar A. Hossain2,3, Tofazzal Islam4, Yusuke Yamauchi2,3,5,6, Kevin C.-W. Wu1 1Department of Chemical Engineering, National Taiwan University, 2International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 3Australian Institute for Innovative Materials (AIIM), University of Wollongong, 4Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, 5School of Chemical Engineering & Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, 6Department of Plant and Environmental New Resources, Kyung Hee University We present a protocol for the synthesis of C5 sugars (xylose and arabinose) from a renewable non-edible lignocellulosic biomass (i.e., jute) with the presence of Brønsted acidic ionic liquids (BAILs) as the catalyst in water. The BAILs catalyst exhibited better catalytic performance than conventional mineral acid catalysts (H2SO4 and HCl).