AnSteel Group General Hospital 1 article published in JoVE Immunology and Infection Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma Guo Hong*1,2, Shuangyi Fan*3, The Phyu3, Priyanka Maheshwari2, Michal Marek Hoppe2, Hoang Mai Phuong2, Sanjay de Mel4, Michelle Poon4, Siok-Bian Ng2,3, Anand D. Jeyasekharan2,4 1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.