Fudan University View Institution's Website 29 articles published in JoVE Engineering Membraneless Hydrogen Peroxide Fuel Cells as a Promising Clean Energy Source Fenyang Zhu1, Guoxiang Chen1, Aleksei Kuzin1,2, Dmitry A. Gorin2, Brij Mohan3, Gaoshan Huang1, Yongfeng Mei1,4,5,6,7, Alexander A. Solovev1 1Department of Materials Science & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, 2Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, 3Centro de Quimica Estrutural, Institute of Molecular Sciences, Instituto Superior Tecnico, Universidade de Lisboa, 4Center for Biomedical Engineering, School of Information Science and Technology, Fudan University, 5International Institute of Intelligent Nanorobots and Nanosystems, Fudan University, 6Shanghai Frontiers Science Research Base of Intelligent Optoelectronics and Perception, Fudan University, 7Yiwu Research Institute of Fudan University This protocol introduces the design and evaluation of innovative three-dimensional electrodes for hydrogen peroxide fuel cells, utilizing Au-electroplated carbon fiber cloth and Ni-foam electrodes. The research findings highlight hydrogen peroxide's potential as a promising candidate for sustainable energy technologies. Neuroscience Abdominal Massage to Improve Motor Dysfunction in Rats with Cerebral Palsy Rui Qiao1, Ayipaxiaguli Kasimu2, Danmei Chen2, Chao Gao3, Bing Li2 1Yunnan University of Traditional Chinese Medicine, 2Department of Traditional Chinese Medicine, Jinshan Hospital of Fudan University, Fudan University, 3Children’s Hospital Affiliated with Zhengzhou University Here, we present a set of massage manipulations in the rat model of cerebral palsy that can considerably improve the motor function of cerebral palsy rats. Biology Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis Chun Wang*1, Jie Lv*1, Wei Zhuang1,2, Ling Xie1, Guangyu Liu1, Kaidireya Saimaier1, Sanxing Han1, Changjie Shi1, Qiuhong Hua1, Ru Zhang1, Guangfeng Shi3, Changsheng Du1 1Putuo People's Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, 2National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 3Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University The present protocol describes the induction of experimental autoimmune encephalomyelitis in a mouse model using myelin oligodendrocyte glycoprotein and monitoring the disease process using a clinical scoring system. Experimental autoimmune encephalomyelitis-related symptoms are analyzed using mouse femur micro-computed tomography analysis and open field test to assess the disease process comprehensively. Medicine Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method Xiaoli Jin*1, Yang Li*2, Xuanwei Chen1, Jin Chen1, Jian Xu1 1School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 2School of Basic Medical Sciences, Fudan University Here we present a protocol for the isolation of BMMs from SD rats, called the secondary adherence method. Bioengineering Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall Mieradilijiang Abudupataer*1, Xiujie Yin*1, Bitao Xiang*1, Nan Chen1, Shiqiang Yan2, Shichao Zhu1, Yang Ming1, Gang Liu1, Xiaonan Zhou1, Hao Lai1, Chunsheng Wang1, Kai Zhu1, Jun Li1 1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University Here, we developed a human aorta smooth muscle cell organ-on-a-chip model to replicate the in vivo biomechanical strain of smooth muscle cells in the human aortic wall. Neuroscience A Mouse Model of Direct Anastomosis via the Prespinal Route for Crossing Nerve Transfer Surgery Zhengrun Gao*1,2, Gaowei Lei*1, Zhen Pang*1, Yiming Chen1, Shuai Zhu1, Kun Huang1, Weishan Lin1, Yundong Shen1,2,3,4,5, Wendong Xu1,2,3,4,5 1Department of Hand Surgery, Huashan Hospital, Fudan University, 2Department of Hand and Upper Extremity Surgery, Jing‘an District Central Hospital, Fudan University, 3The National Clinical Research Center for Aging and Medicine, Fudan University, 4Institutes of Brain Science, Fudan University, 5State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center of Brain Science, Fudan University We simulated clinical surgery to establish a protocol of direct anastomosis of bilateral brachial plexus nerves via the prespinal route in mice, contributing to the study of the neural mechanisms underlying rehabilitation upon crossing nerve transfer after central and peripheral nervous system injuries. Medicine Preparation of Naringenin Solution for In Vivo Application Shufen Liu1, Jingcheng Dong2, Qin Bian2 1Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Department of Integrative Medicine, Huashan Hospital, Fudan University Here, the protocol presents the preparation of naringenin solution for in vivo intraperitoneal administration. Naringenin is fully dissolved in a mixture of dimethylsulfoxide, Tween 80, and saline. The antidiabetic osteoporotic effects of naringenin were assessed by blood glucose testing, tartrate-resistant acid phosphatase staining, and enzyme-linked immunosorbent assay. Medicine Endaural Endoscopic Atticoantrotomy (Retrograde Mastoidectomy) using a Constant Suction Bone-drilling Technique Zhen Gao*1,2,3, Xian-Hao Jia*1,2,3, Ya-Sheng Yuan1,2,3 1Department of Otology and Skull Base Surgery, Eye & ENT Hospital, Fudan University, 2Shanghai Auditory Medical Center, 3NHC Key Laboratory of Hearing Medicine (Fudan University) In this paper, we present the protocol and short-term outcomes of endaural exclusive endoscopic atticoantrotomy using a constant suction bone-drilling technique. Biology A Mouse Model of Lumbar Spine Instability Shufen Liu1, Yueli Sun1, Jingcheng Dong2, Qin Bian2 1Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Department of Integrative Medicine, Huashan Hospital, Fudan University We developed a lumbar intervertebral disc degeneration mouse model by resection of L3–L5 spinous processes along with supra- and inter-spinous ligaments and detachment of paraspinous muscles. Neuroscience Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting Zarmeen Mussa1,2, Jessica Tome-Garcia1,2, Yan Jiang3, Schahram Akbarian2,4, Nadejda M. Tsankova1,2 1Department of Pathology, Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science, Fudan University, 4Department of Psychiatry, Icahn School of Medicine at Mount Sinai We have developed a method that enriches for and isolates human astrocyte populations from fresh-frozen tissue for use in downstream molecular analyses. Behavior A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice Yi Xie1, Sai-Yue Deng1, Si-Miao Chen1, Xue-Jiao Chen1, Wen-Wen Lai1, Li-Fang Huang1, Li Ba1, Wei Wang1,2, Feng-Fei Ding1,3 1Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China, 2Key Laboratory of Neurological Diseases of Chinese Ministry of Education, The School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000,China, 3Department of Pharmacology, School of Basic Medical Science; State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China Presented here is a protocol for chronic sleep fragmentation (CSF) model achieved by an electrically controlled orbital rotor, which could induce confirmed cognitive deficit and anxiety-like behavior in young wild-type mice. This model can be applied to explore the pathogenesis of chronic sleep disturbance and related disorders. Cancer Research Transradial Access Chemoembolization for Hepatocellular Carcinoma Patients Nan Du1,2, Jingqin Ma1,2, Minjie Yang1,2, Zihan Zhang1,2, Zhiyuan Zheng1,2, Wen Zhang*1,2, Zhiping Yan*1,2 1Department of Interventional Radiology, Zhongshan Hospital, Fudan University, 2Shanghai Institution of Medical Imaging Transarterial chemoembolization (TACE) is the standard therapy for patients in the intermediate stage of hepatocellular carcinoma and is typically performed through femoral artery access. Compared with transfemoral access, transradial access (TRA) can decrease the rate of bleeding complications and improve patient tolerance. A method is presented here to perform transarterial chemoembolization via radial artery access. Bioengineering Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution Nan Chen1,2, Kai Zhu1,2, Shiqiang Yan3,4, Junmin Li1,2, Tianyi Pan4, Mieradilijiang Abudupataer1,2, Fazle Alam4, Xiaoning Sun1,2, Li Wang3,4, Chunsheng Wang1,2 1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Shanghai Institute of Cardiovascular Diseases, 3Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 4Institutes of Biomedical Sciences and Department of Systems Biology for Medicine, Shanghai Medical College, Fudan University Here, we developed a novel multilayered modified strategy for liquid-like bioinks (gelatin methacryloyl with low viscosity) to prevent the sedimentation of encapsulated cells. Cancer Research Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment Lei Wang*1, Huan Chen*2,3, Fei Fei*1, Xianfeng He2,3, Shaoyang Sun1, Kunpeng Lv1, Bo Yu2,3, Jiang Long2,4,5,6, Xu Wang1 1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College, Fudan University, 6Pancreatic Cancer Institute, Fudan University This protocol describes optimization procedures in a virus-based dual fluorescence-labeled tumor xenograft model using larval zebrafish as hosts. This heterogeneous xenograft model mimics the tissue composition of pancreatic cancer microenvironment in vivo and serves as a more precise tool for assessing drug responses in personalized zPDX (zebrafish patient-derived xenograft) models. Immunology and Infection Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy Liangfei Niu1, Cong Wang1, Kaile Zhang1,2, Miaomiao Kang1,2, Rui Liang1, Xiaonan Zhang1, Bo Yan1 1Shanghai Public Health Clinical Center, Fudan University, 2School of Life Sciences, Bengbu Medical College This protocol describes a technique for visualizing macrophage behavior and death in embryonic zebrafish during Mycobacterium marinum infection. Steps for the preparation of bacteria, infection of the embryos, and intravital microscopy are included. This technique may be applied to the observation of cellular behavior and death in similar scenarios involving infection or sterile inflammation. Medicine Three-Dimensional Printing of a Complex Aortic Anomaly Xiaoning Sun1,2, Kai Zhu1,2, Weijia Zhang1,2,3,4, Hongqiang Zhang1,2, Fazong Hu5, Chunsheng Wang1,2 1Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, 2Shanghai Institute of Cardiovascular Disease, 3State Key Laboratory of Molecular Engineering of Polymers, Fudan University, 4Institutes of Biomedical Sciences, Fudan University, 5Meditool Shanghai Enterprise Co., Ltd. Here, we present a protocol to use three dimensional printed models for pre-operative planning and intra-operative reorganization of complicated vascular locations when handling a congenital aortic anomaly. Immunology and Infection Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation Jun-O Jin1,2, Soyeong Jang3, Hyehyun Kim4, Junghwan Oh4, Sungbo Shim5, Minseok Kwak3,4, Peter C.W. Lee6 1Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, 2Department of Medical Biotechnology, Yeungnam University, 3Department of Chemistry, Pukyong National University, 4Marine-integrated Bionics Research Center, Pukyong National University, 5Department of Biochemistry, Chungbuk National University, 6Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center Experimental procedures for the subsequent extraction of lymphatic tissues to test lymphoid dendritic cell activation are described after treatment of an immunostimulating nanomaterial. Cancer Research Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation Juan Wang1, Jin Wen2, Xiao-Xiang Chen1, Guang-Liang Chen3 1Department of Rheumatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 2Department of Nephrology and Rheumatology, Yongchuan Hospital of Chongqing Medical University, 3Department of Medical Oncology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University Here, we present a reliable and straightforward two-dimensional (2D) coculture system for studying the interaction between tumor cells and bone marrow adipocytes, which reveals a dual effect of melanoma cell-derived factors on the bone marrow adipocytes differentiation and also poses a classic method for the mechanistic study of bone metastasis. Medicine Endoscopic Septoplasty with Limited Two-line Resection: Minimally Invasive Surgery for Septal Deviation Ke-Qing Zhao1, Shi-Lei Pu1,2, Hong-Meng Yu1 1Department of Otorhinolaryngology-Head and Neck Surgery, Eye and Ear, Nose, and Throat (ENT) Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University, 2 Endoscopic septoplasty is a time-honored surgical procedure with multiple variations. This paper focuses on a step-by-step surgical approach to perform a modified septoplasty procedure known as limited two-line resection. This surgical technique can be applied to correct a deviated nasal septum in the absence of an external nasal deformity. Biology An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm Ke-Qing Zhao*1, Yang Wu*2, Yu-Xi Yi2, Si-Jia Feng2, Ruo-Yan Wei2, Ying Ma3, Chun-Quan Zheng1, Di Qu2 1Department of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, Shanghai Key Clinical Disciplines of otorhinolaryngology, Fudan University, 2Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Department of Medical Microbiology and Parasitology, School of Basic Medical Science, Shanghai Medical College of Fudan University, 3Department of Dermatology, Huashan Hospital, Fudan University This manuscript describes a protocol to study the antimicrobial effect of 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) on a Staphylococcus aureus biofilm. This protocol can be used to develop an in vitro model to study the treatment of bacterial biofilms with PDT in the future. Cancer Research A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay Ying Wang1, DanQi Chen1, MingYu Chen1, KaiYuan Ji1, DeXuan Ma1, LiangFu Zhou1 1Department of Neurosurgery, Huashan Hospital, Fudan University This paper presents a comprehensive procedure to evaluate in vitro whether classic tumor angiogenesis exists in hemangioblastomas (HBs) and its role in HBs. The results highlight the complexity of HB-neovascularization and suggest that this common form of angiogenesis is only a complementary mechanism in the HB-neovascularization. Neuroscience Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System Weiguang Weng1, Dongxue Li1, Cheng Peng1, Thomas Behnisch1 1The Institutes of Brain Science, the State Key Laboratory of Medical Neurobiology, the Collaborative Innovation Center for Brain Science, Fudan University This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices. Neuroscience An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury Chen Chen1,2, Yi Ping Zhang3, Yan Sun1,4, Wenhui Xiong1, Lisa B. E. Shields3, Christopher B. Shields3,5, Xiaoming Jin1, Xiao-Ming Xu1 1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, and Department of Neurological Surgery, Indiana University School of Medicine, 2Program in Medical Neuroscience, Stark Neurosciences Research Institute, Indiana University School of Medicine, 3Norton Neuroscience Institute, Norton Healthcare, 4Department of Human Anatomy & Histoembryology, School of Basic Medical Sciences, Fudan University, 5Department of Neurological Surgery, University of Louisville School of Medicine We introduce an in vivo imaging method using two different fluorescent dyes to track dynamic spinal vascular changes following a contusive spinal cord injury in adult Sprague-Dawley rats. Biology Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary Wei Hu*1, Amin Tamadon*1, Aaron J.W. Hsueh2, Yi Feng1 1Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University, 2Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries. Biology In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells Fengxi Meng*1,2, Xiaofeng Li*1,2, Hui Ren1,2, Jiang Qian1,2 1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University Small ubiquitin-related modifier (SUMO) family proteins are conjugated to the lysine residues of target proteins to regulate various cellular processes. This paper describes a protocol for the detection of retinoblastoma (Rb) protein SUMOylation under endogenous and exogenous conditions in human cells. Developmental Biology Dissection of Larval Zebrafish Gonadal Tissue Xinjian Wang*1, Sijie Chen*1, Wei Zhang1, Yiyuan Ren1, Quan Zhang1, Gang Peng1 1Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance. Engineering Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors Jiang Yang1,2, Tae-Joon Kwak3, Xiaodong Zhang4, Robert McClain5, Woo-Jin Chang*3,6, Sundaram Gunasekaran*1 1Department of Biological Systems Engineering, University of Wisconsin-Madison, 2Environment, Energy and Natural Resources Center, Department of Environmental Science and Engineering, Fudan University, 3Department of Mechanical Engineering, University of Wisconsin-Milwaukee, 4Department of Physics, School of Science, Tianjin University, 5Department of Chemistry, University of Wisconsin-Madison, 6School of Freshwater Sciences, University of Wisconsin-Milwaukee The study demonstrates the growth of iridium oxide-reduced graphene oxide (IrO2-RGO) nanohybrid thin films on irregular and rough screen-printed carbon substrate through a green electrochemical synthesis, and their implementation as a pH sensor with a patterned paper-fluidic platform. Medicine Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy Yan Wang1,3,4, Dale P. Brown1, Brant D. Watson2, Jeffrey L. Goldberg1,3 1Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 2Departments of Neurology and Biomedical Engineering, University of Miami Miller School of Medicine, 3Shiley Eye Center, University of California, 4Department of Ophthalmology and Vision Science, Fudan University The goal of this protocol is to photochemically induce ischemic injury to the posterior optic nerve in rat. This model is critical to studies of the pathophysiology of posterior ischemic optic neuropathy, and therapeutic approaches for this and other optic neuropathies, as well as of other CNS ischemic diseases. Biology Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) Shuang Hou*1,2,3, Duy Linh Phung*1,2,3, Wei-Yu Lin1,2,3, Ming-wei Wang4, Kan Liu5, Clifton Kwang-Fu Shen1,2,3 1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, 2Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, 3California NanoSystems Institute, University of California at Los Angeles, 4Nuclear Medicine, PET Center, Shanghai Medical Collegea, Fudan University, 5Electronics and Information Engineering, College of Electronics and Information Engineering, Wuhan Textile University A facile, one-pot synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) was developed based on a non-aqueous, three-step radiochemical process. Using microwave heating, the entire procedure can be completed in less than 30 min, or 60 min with further purification by preparative HPLC. The decay-corrected radiochemical yields (RCYs) were 35-5% (n > 30).