AIT Austrian Institute of Technology GmbH 1 article published in JoVE Chemistry Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures Yasaman Ahmadi1, Ivan Barisic1 1Molecular Diagnostics, Centre for Health and Bioresources, AIT Austrian Institute of Technology GmbH Here, a protocol for the protection of DNA origami nanostructures in Mg-depleted and nuclease-rich media using natural cationic polysaccharide chitosan and synthetic linear polyethyleneimine (LPEI) coatings is presented.