Johannes Kepler University Linz 1 article published in JoVE Cancer Research Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells Eva Dovjak1, Mario Mairhofer1, Claudia Wöß1, Jingjing Qi1, Dorothy N. Y. Fan2, Clemens A. Schmitt1,2,3, Yong Yu1 1Johannes Kepler University Linz, 2Charité - University Medicine Berlin, 3Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association Here we present a flow cytometry-based method for visualization and quantification of multiple senescence-associated markers in single cells.