Labdeers s.r.o 1 article published in JoVE Biology Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation Veronika Balakhonova*1,2, Nadiia Pushkarova*1,3, Jan Skalak1,2, Tereza Dobisova1,2,4, Zuzana Benedikty5, Klara Panzarova5, Martin Trtilek5, Jan Hejátko1,2 1CEITEC - Central European Institute of Technology, Masaryk University, 2National Centre for Biomolecular Research, Faculty of Science, Masaryk University, 3Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, 4Labdeers s.r.o, 5Photon Systems Instruments, Prumyslova Here, we describe an advanced tool designed for chlorophyll biosynthesis monitoring during the early stages of Arabidopsis seedling de-etiolation. The novel methodology provides non-invasive real-time chlorophyll fluorescence imaging at high spatial and temporal resolution.