University of Konstanz View Institution's Website 7 articles published in JoVE Medicine A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection Hang You1,2, Xin Mao2, Chenyang Wang2, Gang Huang3, Marcus Groettrup4,5, Jun Li2 1School of Medicine, Chongqing University, 2Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University, 4Division of Immunology, Department of Biology, University of Konstanz, 5Biotechnology Institute Thurgau, University of Konstanz The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis. Developmental Biology A Simple and Effective Transplantation Device for Zebrafish Embryos Gary H. Soh1, Anna C. Kögler1, Patrick Müller1,2 1Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society, 2University of Konstanz Embryological manipulations such as extirpation and transplantation of cells are important tools to study early development. This protocol describes a simple and effective transplantation device to perform these manipulations in zebrafish embryos. Neuroscience Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera Eirik Søvik*1,2, Jenny A. Plath*3,4, Jean-Marc Devaud5, Andrew B. Barron3 1Department of Science and Mathematics, Volda University College, 2Department of Biology, Washington University in St. Louis, 3Department of Biological Sciences, Macquarie University, 4Department of Biology, University of Konstanz, 5Research Center on Animal Cognition, CNRS, Universite de Toulouse This manuscript describes several protocols for administering pharmacological agents to honey bees, including simple noninvasive methods for free-flying bees, as well as more invasive variants that allow precise localized treatment of restrained bees. Chemistry In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging Martin Spitzbarth1, Tobias Lemke1, Malte Drescher1 1Department of Chemistry, Universität Konstanz A protocol for the in situ monitoring of the diffusion of guest molecules in porous media using electron paramagnetic resonance (EPR) imaging is presented. Developmental Biology Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation Vaibhav Shinde*1, Stefanie Klima*2, Perumal Srinivasan Sureshkumar1, Kesavan Meganathan1, Smita Jagtap1, Eugen Rempel3, Jörg Rahnenführer3, Jan Georg Hengstler4, Tanja Waldmann2, Jürgen Hescheler1, Marcel Leist*2, Agapios Sachinidis*1 1Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, 2Department of Biology, University of Konstanz, 3Department of Statistics, Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund The protocols describe two in vitro developmental toxicity test systems (UKK and UKN1) based on human embryonic stem cells and transcriptome studies. The test systems predict human developmental toxicity hazard, and may contribute to reduce animal studies, costs and the time required for chemical safety testing. Neuroscience Appetitive Associative Olfactory Learning in Drosophila Larvae Anthi A. Apostolopoulou1, Annekathrin Widmann1, Astrid Rohwedder1,2, Johanna E. Pfitzenmaier1, Andreas S. Thum1,2 1Department of Biology, University of Konstanz, 2Department of Biology, University of Fribourg Drosophila larvae are able to associate odor stimuli with gustatory reward. Here we describe a simple behavioral paradigm that allows the analysis of appetitive associative olfactory learning. Neuroscience Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe Ana F. Silbering1, Rati Bell1, C. Giovanni Galizia2, Richard Benton1 1Center for Integrative Genomics, University of Lausanne, 2Department of Biology, University of Konstanz We describe an established technique to measure and analyze odor-evoked calcium responses in the antennal lobe of living Drosophila melanogaster.