University Medical Center 1 article published in JoVE Medicine Multicolor 3D Printing of Complex Intracranial Tumors in Neurosurgery Michael Kosterhon1, Meik Neufurth2, Axel Neulen1, Lea Schäfer1, Jens Conrad1, Sven R. Kantelhardt1, Werner E. G. Müller2, Florian Ringel1 1Department of Neurosurgery, University Medical Center of the Johannes Gutenberg University Mainz, Germany, 2Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Germany The protocol describes the fabrication of fully colored three-dimensional prints of patient-specific, anatomical skull models to be used for surgical simulation. The crucial steps of combining different imaging modalities, image segmentation, three-dimensional model extraction, and production of the prints are explained.