PROiSER R D S.L. 1 article published in JoVE Biology Fish Sperm Assessment Using Software and Cooling Devices Carina Caldeira1, Carles Soler1,2 1PROiSER R+D S.L., 2Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia The present protocol describes a procedure of fish sperm assessment using computer-assisted sperm analysis and cooling devices. The software gives a rapid, accurate and quantitative analysis of fish sperm quality based on spermatozoa motility, which can be a useful tool in aquaculture to improve reproduction success.