CIBERONC 1 article published in JoVE Cancer Research Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer Irene Chicote1, Jordi Martínez-Quintanilla1, Juan Antonio Cámara2, Héctor G. Palmer1,3 1 This protocol describes the orthotopic implantation of patient-derived cancer cells in the cecum wall of immunodeficient mice. The model recapitulates advanced colorectal cancer metastatic disease and allows for the evaluation of new therapeutic drugs in a clinically relevant scenario of lung and liver metastases.