University of Zaragoza-IACS 1 article published in JoVE Cancer Research Transmitochondrial Cybrid Generation Using Cancer Cell Lines Ruth Soler-Agesta1, Joaquín Marco-Brualla1, Patricio Fernández-Silva1,2, Pilar Mozas1,3, Alberto Anel1, Raquel Moreno Loshuertos1,2 1Department of Biochemistry, University of Zaragoza, 2Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza, 3Sequencing and Functional Genomics Unit, University of Zaragoza-IACS This protocol describes a technique for cybrid generation from suspension-growing cancer cells as a tool to study the role of mitochondria in the tumorigenic process.