Hospices-Civils-de-Lyon, Centre Hospitalier Lyon-Sud 1 article published in JoVE Medicine Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics Marion Gilormini1, Anne-Sophie Wozny1,2, Priscillia Battiston-Montagne1, Dominique Ardail1,2, Gersende Alphonse1,2, Claire Rodriguez-Lafrasse1,2 1UCBL1, UMR/CNRS 5822, Laboratoire de Radiobiologie Cellulaire Moléculaire, Université de Lyon, 2Hospices-Civils-de-Lyon, Centre Hospitalier Lyon-Sud Understanding the role of cancer stem-like cells in tumor recurrence and resistance to therapies has become a topic of great interest in the last decade. This article describes the isolation and characterization of the sub-population of cancer stem-like cells from head and neck squamous carcinoma cell lines (HNSCC).