AstraZeneca 3 articles published in JoVE Medicine Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis Paola Marco-Casanova1, Natalia Lukashchuk1, Benedetta Lombardi1, Veerendra Munugalavadla2, Melanie M. Frigault3, Elizabeth A. Harrington1, J. Carl Barrett3, Andrew J. Pierce1 1Translational Medicine, R&D Oncology, AstraZeneca, 2Acerta Pharma, AstraZeneca Group, 3Translational Medicine, R&D Oncology, AstraZeneca This protocol details clinically implementable preparation of high quality PBMC and plasma biosamples at the clinical trial site that can be used for translational biomarker analysis. Genetics An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level Payam Haftbaradaran Esfahani1, Ralph Knöll1,2 1Department of Medicine, Integrated Cardio Metabolic Centre (ICMC), Heart and Vascular Theme, Karolinska Institutet, 2Bioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca This paper presents methods for growing cardiac myocytes with different shapes, which represent different pathologies, and sorting these adherent cardiac myocytes based on their morphology at a single cell level. The proposed platform provides a novel approach to high throughput and drug screening for different types of heart failure. Chemistry A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy Zhong Wang1, Benjamin F. Jeffries1, Hannah R. Felstead1, Neil J. Wells1, Elisabetta Chiarparin2, Bruno Linclau1 1Chemistry, University of Southampton, 2Medicinal Chemistry, Oncology, IMED Biotech Unit, AstraZeneca A novel and straightforward variation of the shake-flask method was developed for accurate lipophilicity measurement of fluorinated compounds by 19F NMR spectroscopy.