The Institute of Cancer Research: and the Royal Marsden NHS Foundation Trust 1 article published in JoVE Cancer Research Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer Kaitriana E. Colling*1,2, Emily L. Symons*1, Lorenzo Buroni*3, Hiruni K. Sumanasiri1, Jessica Andrew-Udoh1, Emily Witt1,4, Haley A. Losh1, Abigail M. Morrison1, Kimberly K. Leslie5, Christopher J. Dunnill6, Johann S. de Bono3, Kristina W. Thiel1,7 1Department of Obstetrics and Gynecology, Carver College of Medicine, University of Iowa, 2Cancer Biology Graduate Program, Carver College of Medicine, University of Iowa, 3The Institute of Cancer Research: and the Royal Marsden NHS Foundation Trust, 4Department of Radiation Oncology, Carver College of Medicine, University of Iowa, 5Division of Molecular Medicine, Departments of Internal Medicine and Obstetrics and Gynecology, University of New Mexico Comprehensive Cancer Center, University of New Mexico Health Sciences Center, 6Agilent Technologies, 7Holden Comprehensive Cancer Center, University of Iowa Patient-derived tumor organoids are a sophisticated model system for basic and translational research. This methods article details the use of multiplexed fluorescent live-cell imaging for simultaneous kinetic assessment of different organoid phenotypes.
