Nuovo Ospedale Santo Stefano 1 article published in JoVE Medicine An “All-laser” Endothelial Transplant Francesca Rossi1, Annalisa Canovetti2, Alex Malandrini2, Ivo Lenzetti2, Roberto Pini1, Luca Menabuoni2 1Institute of Applied Physics, Italian National Research Council, 2U.O. Oculistica, Nuovo Ospedale Santo Stefano An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.