University of Napoli Federico II 1 article published in JoVE Environment Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution Giovanni Gallo1,2, Martina Aulitto1,3, Patrizia Contursi1,4, Danila Limauro1,4, Simonetta Bartolucci1, Gabriella Fiorentino1,4 1Department of Biology, University of Naples Federico II, 2Institute of Polymers, Composites and Biomaterials (IPCB), Consiglio Nazionale delle Ricerche CNR, 3Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, 4BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli Federico II The isolation of heavy metal-resistant microbes from geothermal springs is a hot topic for the development of bioremediation and environmental monitoring biosystems. This study provides a methodological approach for isolating and identifying heavy metal tolerant bacteria from hot springs.