Royal Netherlands Academy of Arts & Sciences (KNAW) View Institution's Website 2 articles published in JoVE Neuroscience Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells Kimberly L. Pietersz1, Paul J.H. Nijhuis1, Matthijs H.M. Klunder1, Joëlle van den Herik1, Barbara Hobo1, Fred de Winter1, Joost Verhaagen1,2 1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam Here, a suspension HEK293 cell-based AAV production protocol is presented, resulting in reduced time and labor needed for vector production using components that are available for research purposes from commercial vendors. Neuroscience Video-oculography in Mice Marcel de Jeu1, Chris I. De Zeeuw1,2 1Department of Neuroscience, Erasmus MC, Rotterdam, The Netherlands, 2Department of Neuroscience, Royal Dutch Academy of Arts & Sciences (KNAW) Video-oculography is a very quantitative method to investigate ocular motor performance as well as motor learning. Here, we describe how to measure video-oculography in mice. Applying this technique on normal, pharmacologically-treated or genetically modified mice is a powerful research tool to explore the underlying physiology of motor behaviors.
