Miltenyi Biotec GmbH View Institution's Website 6 articles published in JoVE Medicine Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells David J. Agorku1, Stefan Tomiuk1, Kerstin Klingner2, Stefan Wild1, Silvia Rüberg1, Lisa Zatrieb1, Andreas Bosio1, Julia Schueler2, Olaf Hardt1 1R&D Reagents, Miltenyi Biotec GmbH, 2In vivo Tumorbiology, Oncotest GmbH Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting. Biology Generation of Single-Cell Suspensions from Mouse Neural Tissue Sandra Pennartz1, Sandy Reiss1, Rebecca Biloune1, Doris Hasselmann1, Andreas Bosio1 1Miltenyi Biotec,GmbH Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained. Biology Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator Melanie Jungblut1, Karen Oeltze1, Irene Zehnter1, Doris Hasselmann1, Andreas Bosio1 1Miltenyi Biotec,GmbH Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained. Biology Detection and Isolation of Viable Mouse IL-17-Secreting T Cells Anna Foerster1, Mario Assenmacher1, Michaela Niemoeller1, Elly Rankin1, Mariette Mohaupt1, Anne Richter1 1Miltenyi Biotec,GmbH This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation. Biology Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator Melanie Jungblut1, Karen Oeltze1, Irene Zehnter1, Doris Hasselmann1, Andreas Bosio1 1Miltenyi Biotec,GmbH This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes. Application Notes gentleMACS Dissociatorを用いたマウス脾臓の単細胞懸濁液の調製方法 - ADVERTISEMENT Melanie Jungblut1, Karen Oeltze1, Irene Zehnter1, Doris Hasselmann1, Andreas Bosio1 1Miltenyi Biotec,GmbH