Cultex Laboratories GmbH 1 article published in JoVE Medicine Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface Amelie Tsoutsoulopoulos1, Katrin Gohlsch2, Niklas Möhle3, Andreas Breit2, Sebastian Hoffmann5, Olaf Krischenowski3,4, Harald Mückter2, Thomas Gudermann2, Horst Thiermann1, Michaela Aufderheide3,4, Dirk Steinritz1,2 1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology, University of Munich, 3Cultex Laboratories GmbH, 4Cultex Technologies GmbH (formerly Cultex Laboratories GmbH), 5seh consulting + services We present a robust, transferable and predictive in vitro exposure system for the screening and monitoring of airborne particles concerning their acute pulmonary cytotoxicity by exposing cultivated human lung cells at the air-liquid interface (ALI).