Algoma University 1 article published in JoVE Biology Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers Seamus Jenkin1, Isabel Molina1 1Department of Biology, Algoma University Lipid polyesters constitute the structural components of two cell wall modifications, the plant cuticle and suberin-containing diffusion barriers. In this video, we describe a method to depolymerize cutin from whole delipidated leaves. The method can be applied to investigating mutants compromised in either cutin or suberin biosynthesis.