Universidad Nacional Autonoma de Mexico (UNAM) 3 articles published in JoVE Biology Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples Eduardo Delgadillo-Tellez1, Beatriz Zavaleta-Villa1, Daniel Atilano-López2, Rigoberto Hernández-Castro3, Angélica Olivo-Díaz1, Erika Margarita Carrillo-Casas1 1Department of Molecular Biology and Histocompatibility, General Hospital “Dr. Manuel Gea González”, 2Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty, UNAM, 3Department of Ecology of Pathogens, General Hospital “Dr. Manuel Gea González” In this study, a dot-blot application was designed to detect Leptospira from the three main clades in water samples. This method allows for the identification of minimal DNA quantities specifically targeted by a digoxigenin-labeled probe, easily detected by an anti-digoxigenin antibody. This approach is a valuable and satisfactory tool for screening purposes. Biology Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance Xarubet Ruiz-Herrera1, Ivan Luzardo-Ocampo1, Gonzalo Martínez de la Escalera1, Carmen Clapp1, Yazmín Macotela1 1Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM) This protocol describes the isolation of mouse preadipocytes from subcutaneous fat, their differentiation into mature adipocytes, and the induction of insulin resistance. Insulin action is evaluated by the phosphorylation/activation of members of the insulin signaling pathway through western blot. This method allows direct determination of insulin resistance/sensitivity in primary adipocytes. Medicine Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy Benjamín León-Mancilla1,2, Moisés Martínez-Castillo1, Zaira Medina-Avila1, Armando Pérez-Torres3, Jorge Garcia-Loya2, Ana Alfaro-Cruz4, Cristina Piña-Barba5, Gabriela Gutierrez-Reyes1 1Liver, Pancreas and Motility Laboratory; Unit of Experimental Medicine; School of Medicine, Universidad Nacional Autónoma de Mexico (UNAM), 2Department of Surgery, School of Medicine, Universidad Nacional Autónoma de México (UNAM), 3Department of Cells and Tissue Biology, School of Medicine, Universidad Nacional Autónoma de México (UNAM), 4Department of Pathology, Hospital General de Mexico, 5Materials Research Institute, Universidad Nacional Autónoma de México (UNAM) Liver diseases are induced by many causes that promote fibrosis or cirrhosis. Transplantation is the only option for recovering health. However, given the scarcity of transplantable organs, alternatives must be explored. Our research proposes the implantation of collagen scaffolds in liver tissue from an animal model.
