Universidad Central de Queretaro 1 article published in JoVE Behavior Handling Techniques to Reduce Stress in Mice Michael Marcotte1, Ashley Bernardo1,2, Nathaniel Linga3, Carmina A. Pérez-Romero4, Jean-Louis Guillou5, Etienne Sibille1,2,3, Thomas D. Prevot1,2 1Campbell Family Mental Health Research Institute of CAMH, 2Department of Psychiatry, University of Toronto, 3Department of Pharmacology and Toxicology, University of Toronto, 4Departamento de Investigación, Universidad Central de Queretaro, 5Centre National de la Recherche Scientifique, UMR 5287, Institut de Neurosciences Cognitives et Intégratives d'Aquitaine, Université de Bordeaux This paper describes a handling technique in mice, the 3D-handling technique, which facilitates routine handling by reducing anxiety-like behaviors and presents details on two existing related techniques (tunnel and tail handling).