Carl Hayden Bee Research Center 2 articles published in JoVE Environment Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers Vanessa Corby-Harris1, Lucy A. Snyder1 1USDA-ARS, Carl Hayden Bee Research Center Hypopharyngeal gland acinus size is a robust measure of nurse honey bee nutrition. Here, we provide a detailed protocol for dissecting, staining, imaging, and measuring nurse bee hypopharyngeal gland acini. Biology Monitoring Colony-level Effects of Sublethal Pesticide Exposure on Honey Bees William G. Meikle1, Milagra Weiss1 1Carl Hayden Bee Research Center Sublethal doses of pesticides may affect colonies in ways that are difficult to detect using only periodic or visual methods. Methods to measure total adult bee mass, brood, and food resources by weighing hives and hive parts, photographing frames, and installing sensors, are provided. Data analysis is also addressed.