Roche Sequencing Solutions 1 article published in JoVE Cancer Research Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues Charles A. Havnar1, Oliver Zill2, Jeff Eastham1, Jeffrey Hung1, Manana Javey3, Emmanuel Naouri3, Jennifer Giltnane1, Justin M. Balko4, Andrew Wallace2, Nicolas Lounsbury2, Daniel Oreper2, Sarajane Saturnio1, G-Y Yang5, Amy A. Lo1 1Departments of Research Pathology, Genentech, 2Bioinformatics & Computational Biology, Genentech, 3Roche Sequencing Solutions, Hacienda Drive, 4Department of Medicine, Vanderbilt University Medical Center, Medical Center Drive, 5Department of Pathology, Northwestern University, Feinberg School of Medicine Digital annotation with automated tissue dissection provides an innovative approach to enriching tumor in low tumor content cases and is adaptable to both paraffin and frozen tissue types. The described workflow improves accuracy, reproducibility and throughput and could be applied to both research and clinical settings.