University of Colorado Denver Anschutz Medical Campus 4 articles published in JoVE Bioengineering 3D Bioprinting Phototunable Hydrogels to Study Fibroblast Activation Alicia E. Tanneberger1, Layla Blair1, Duncan Davis-Hall1, Chelsea M. Magin1,2,3 1Department of Bioengineering, University of Colorado Denver | Anschutz Medical Campus, 2Department of Pediatrics, University of Colorado Anschutz Medical Campus, 3Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Anschutz Medical Campus This article describes how to 3D bioprint phototunable hydrogels to study extracellular matrix stiffening and fibroblast activation. Cancer Research Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors Jordi M. Lanis*1, Matthew S. Lewis*1, Hannah Strassburger1, Kristina Larsen1, Stacey M. Bagby2, Adrian T. A. Dominguez2, Juan A. Marín-Jiménez3, Roberta Pelanda1, Todd M. Pitts2, Julie Lang1 1Department of Immunology and Microbiology, School of Medicine, University of Colorado Denver Anschutz Medical Campus, 2Division of Oncology, School of Medicine, University of Colorado Denver Anschutz Medical Campus, 3 This protocol outlines the generation of human immune system (HIS) mice for immuno-oncology studies. Instructions and considerations in the use of this model for testing human immunotherapeutics on human tumors implanted in this model are presented with an emphasis on characterizing the response of the human immune system to the tumor. Medicine Collecting Hair Samples for Hair Cortisol Analysis in African Americans Kathy D. Wright1, Jodi L. Ford1, Joseph Perazzo2, Lenette M. Jones3, Sherrilynn Mahari4, Brent A. Sullenbarger5,6,7, Mark L. Laudenslager8 1College of Nursing, The Ohio State University, 2College of Nursing, University of Cincinnati, 3School of Nursing, University of Michigan, 4Licensed Cosmetologist Consultant, 5Clinical Research Manager, The Ohio State University, 6Biomedical Core Research Coordinator, The Ohio State University, 7College of Nursing Biomedical Core Research Laboratory, The Ohio State University, 8Department of Psychiatry, Behavioral Immunology & Endocrinology Laboratory, University of Colorado Denver Anschutz Medical Campus Hair cortisol concentration analysis provides an alternative to traditional measures of cortisol; however, to collect hair samples from African Americans, scientists need to be culturally informed and competent. The purpose of this protocol is to demonstrate a culturally informed technique to collect hair samples for cortisol analysis from African Americans. Cancer Research Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies Stacey Bagby1, Wells A. Messersmith1, Todd M. Pitts1, Anna Capasso1, Marileila Varella-Garcia1, Peter J. Klauck1, Jihye Kim1, Aik-Choon Tan1, S. Gail Eckhardt1, John J. Tentler1, John Arcaroli1 1Medicine, University of Colorado Denver Anschutz Medical Campus Utilizing patient-derived tumors in a subcutaneous preclinical model is an excellent way to study the efficacy of novel therapies, predictive biomarker discovery, and drug resistant pathways. This model, in the drug development process, is essential in determining the fate of many novel anti-cancer therapies prior to clinical investigation.