LiteCure LLC 1 article published in JoVE Neuroscience A Protocol for Transcranial Photobiomodulation Therapy in Mice Farzad Salehpour1,2, Luis De Taboada3, Paolo Cassano4,5,6, Farzin Kamari1, Javad Mahmoudi1, Sohrab Ahmadi-Kandjani7, Seyed Hossein Rasta8,9,10, Saeed Sadigh-Eteghad1 1Neurosciences Research Center, Tabriz University of Medical Sciences, 2ProNeuroLIGHT LLC, 3LiteCure LLC, 4Department of Psychiatry, Harvard Medical School, 5Depression Clinical and Research Program, Department of Psychiatry, Massachusetts General Hospital, 6Center for Anxiety and Traumatic Stress Disorders, Department of Psychiatry, Massachusetts General Hospital, 7Research Institute for Applied Physics and Astronomy, University of Tabriz, 8Department of Medical Physics, Tabriz University of Medical Sciences, 9Department of Medical Bioengineering, Tabriz University of Medical Sciences, 10School of Medical Sciences, University of Aberdeen Photobiomodulation therapy is an innovative noninvasive modality for the treatment of a wide range of neurological and psychiatric disorders and can also improve healthy brain function. This protocol includes a step-by-step guide to performing brain photobiomodulation in mice by transcranial light delivery, which can be adapted for use in other laboratory rodents.