University of Miami School of Medicine 1 article published in JoVE Neuroscience Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans Bhavya Ravi*1, Layla M. Nassar*1,2, Richard J. Kopchock III*2, Pravat Dhakal*2, Michael Scheetz2, Kevin M. Collins1,2 1Neuroscience Program, University of Miami School of Medicine, 2Department of Biology, University of Miami This protocol describes the use of genetically encoded Ca2+ reporters to record changes in neural activity in behaving Caenorhabditis elegans worms.