USDA 1 article published in JoVE Biology Sieving Fruit Pulp to Detect Immature Tephritid Fruit Flies in the Field Amy L. Roda1, Gary Steck2, Thomas Fezza3, Todd Shelly3, Rita Duncan4, Nicholas Manoukis5, Lori Carvalho5, Abbie Fox6, Paul Kendra7, Daniel Carrillo4 1Animal Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Science and Technology (S&T) Miami, United States Department of Agriculture (USDA), 2Division of Plant Industry, Florida Department of Agriculture and Consumer Services, 3APHIS, PPQ, S&T, USDA, 4IFAS, Tropical Research and Education Center, University of Florida, 5Agricultural Research Service (ARS), Pacific Basin Agricultural Research Center, USDA, 6APHIS, PPQ, Field Operations, USDA, 7ARS, Subtropical Horticulture Research Station, USDA Increasing the detection of immature tephritid fruit flies in the field can trigger timely efforts to eliminate populations of these destructive pests. Detecting late instar larvae is faster and more accurate when mushing host fruit in a bag and passing the pulp through a series of sieves than hand cutting and visual inspection.