National Institute on Drug Abuse 1 article published in JoVE Neuroscience A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals David J. Barker1, David H. Root2, Kevin R. Coffey1, Sisi Ma1, Mark O. West1 1Department of Psychology, Rutgers, the State University of New Jersey, 2Neuronal Networks Section, Integrative Neuroscience Research Branch, National Institute on Drug Abuse Implanting organized arrays of microwires for use in single-unit electrophysiological recordings presents a number of technical challenges. Methods for performing this technique and the equipment necessary are described. Also, the beneficial use of organized microwire arrays to record from distinct neural subregions with high spatial selectivity is discussed.