University of Minnesota Medical School 3 articles published in JoVE Medicine Technical Considerations and Approach to Redo Foregut Surgery David J. Leishman1, Divinemercy Bakare2, Madhuri Rao1 1Department of Surgery, Division of Thoracic and Foregut Surgery, University of Minnesota, 2University of Minnesota Medical School Redo foregut surgery is associated with increased patient morbidity and presents a technical challenge for the surgeon. We describe our approach and considerations when performing a redo hiatal hernia repair to provide a guide for other surgeons and improve patient outcomes. Biology An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus Shane C. McAllister1, Ryan S. Hanson1, Kyleen N. Grissom1, Sara Botto2, Ashlee V. Moses2 1Division of Pediatric Infectious Diseases, University of Minnesota Medical School, 2Vaccine and Gene Therapy Institute, Oregon Health and Science University Kaposi sarcoma (KS) is a tumor induced by infection with the oncogenic virus human herpesvirus-8/KS herpesvirus (HHV-8/KSHV). The endothelial cell culture model described here is uniquely suited for studying the mechanisms by which KSHV transforms host cells. Biology Isolation, Culture, and Transplantation of Muscle Satellite Cells Norio Motohashi1, Yoko Asakura1, Atsushi Asakura1 1Stem Cell Institute, Paul and Sheila Wellstone Muscular Dystrophy Center, Department of Neurology, University of Minnesota Medical School The isolation and culture of a pure population of quiescent satellite cells, a muscle stem cell population, is essential to the understanding of muscle stem cell biology and regeneration, as well as stem cell transplantation for therapies in muscular dystrophy and other degenerative diseases.