Perlmutter Cancer Center 1 article published in JoVE Cancer Research A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis Arman Alberto Sorin Shadaloey1,2, Alcida Karz1,2, Rana S. Moubarak1,2, Praveen Agrawal1,2, Grace Levinson1,2, Kevin Kleffman1,2, Orlando Aristizabal3,4, Iman Osman2,5, Youssef Z. Wadghiri3,4, Eva Hernando1,2 1Department of Pathology, NYU Grossman School of Medicine, 2Interdisciplinary Melanoma Cooperative Group, Perlmutter Cancer Center, 3Department of Radiology, NYU Grossman School of Medicine, 4Preclinical Imaging Core, NYU Grossman School of Medicine, 5Ronald Perelman Department of Dermatology, NYU Grossman School of Medicine This article describes a workflow of techniques employed for testing novel candidate mediators of melanoma metastasis and their mechanism(s) of action.