University Hospitals of Cleveland 2 articles published in JoVE Medicine Development and Functional Characterization of Murine Tolerogenic Dendritic Cells Hsi-Ju Wei1, John J. Letterio2,3, Tej K. Pareek2,3 1Department of Biochemistry, School of Medicine, Case Western Reserve University, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology, Case Western Reserve University, 3Angie Fowler Cancer Institute, Rainbow Babies & Children's Hospital, University Hospitals, Cleveland Here, we present a protocol to develop and characterize tolerogenic dendritic cells (TolDCs) and evaluate their immunotherapeutic utility. Medicine Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases Maneesh Dave1, Paola Menghini1, Keiki Sugi2,3, Rodrigo A. Somoza5, Zhenghong Lee4, Mukesh Jain2,3, Arnold Caplan5, Fabio Cominelli1 1Division of Gastroenterology and Liver Disease, University Hospitals, Digestive Health Research Institute, Case Western Reserve University, 2Case Cardiovascular Research Institute, School of Medicine, Case Western Reserve University, 3Department of Medicine, Harrington Discovery Institute, Harrington Heart and Vascular Institute, University Hospitals Case Medical Center, 4Department of Radiology, University Hospitals Case Medical Center, 5Department of Biology - Skeletal Research Center, Case Western Reserve University Murine studies in models of colonic inflammation have demonstrated that a small percentage (1 - 5%) of mesenchymal stem cells (MSC) injected intravenously or intraperitoneally home to the inflamed colon1,2. This study shows that ultrasound-guided intracardiac injections of MSCs result in increased localization to the intestine.