Portland Veterans Affairs Medical Center 1 article published in JoVE Biology Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy Katsuyuki Matsushita1, Kirsti Golgotiu1, Daniel J. Orton2, Richard D. Smith2, Karin D. Rodland2, Paul D. Piehowski2, Michael P. Hutchens1,3 1Anesthesiology & Perioperative Medicine, Oregon Health & Science University, 2Environmental and Biological Services Division, Pacific Northwest National Laboratory, 3Operative Care Division, Portland Veterans Affairs Medical Center We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.