Janssen Research and Development, LLC 5 articles published in JoVE Immunology and Infection A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model Brian Jones1, Shannon Hitchcock1, Matthew Loza1, Ravi Malaviya1, Tatiana Ort1, Fang Shen1 1Immunology, Janssen Research & Development, Inc. A cryogenic pulverization method to process murine paws using a liquid nitrogen freezer mill was developed to improve the yield and quality of RNA or protein extracted from the tissues and enable the analysis of molecular profiles associated with inflammatory responses. Neuroscience Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia Donata De Marco1, Renske Taggenbrock2,3, Rosa Crespo3, Wouter Koudstaal3, Elizabeth Ramsburg4, Adrian Apetri3 1Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies. Immunology and Infection Antigenic Liposomes for Generation of Disease-specific Antibodies Kyle J. Bednar*1, Lakeya Hardy*2,3, Johanna Smeekens*3,4, Dharmendra Raghuwanshi5, Shiteng Duan6, Mike D. Kulis3,4, Matthew S. Macauley5,7 1Janssen R&D, 2Department of Microbiology and Immunology, University of North Carolina, 3UNC Food Allergy Initiative, University of North Carolina, 4Department of Pediatrics, University of North Carolina, 5Department of Chemistry, University of Alberta, 6Department of Molecular Medicine, Scripps Research Institute, 7Department of Medical Microbiology and Immunology, University of Alberta Described is the preparation of antigenic liposomal nanoparticles and their use in stimulating B-cell activation in vitro and in vivo. Consistent and robust antibody responses led to the development of a new peanut allergy model. The protocol for generating antigenic liposomes can be extended to different antigens and immunization models. Cancer Research Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia Jacqueline Cloos*1,2, Jeffrey R. Harris*3, Jeroen J.W.M. Janssen1, Angele Kelder1, F. Huang3, Gerrit Sijm1, Maike Vonk1, Alexander N. Snel1, Jennifer R. Scheick1, Willemijn J. Scholten1, Jannemieke Carbaat-Ham1, Dennis Veldhuizen1, Diana Hanekamp1, Yvonne J.M. Oussoren-Brockhoff1, Gertjan J.L. Kaspers2,4, Gerrit J. Schuurhuis1, A. Kate Sasser3, Gert Ossenkoppele1 1Department of Hematology, VU University Medical Center, 2Pediatric Oncology/Hematology, VU University Medical Center, 3Janssen Research & Development, LLC, 4Princess Máxima Center for Pediatric Oncology Detection of minimal or measurable residual disease (MRD) is an important prognostic biomarker for refining risk assessment and predicting relapse in acute myeloid leukemia (AML). These comprehensive guidelines and recommendations with best practices for consistent and accurate identification and detection of MRD, may aid in making effective AML treatment decisions. Biochemistry Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT) Christina D. King1, Joseph D. Dudenhoeffer1, Liqing Gu2, Adam R. Evans3, Renã A. S. Robinson1 1Department of Chemistry, University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development Combined precursor isotopic labeling and isobaric tagging (cPILOT) is a quantitative proteomics strategy that enhances sample multiplexing capabilities of isobaric tags. This protocol describes the application of cPILOT to tissues from an Alzheimer's disease mouse model and wild-type controls.