Wright Labs, LLC 1 article published in JoVE Environment Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures Jeremy R. Chen See1,2, Olivia Wright1, Lavinia V. Unverdorben1,2, Nathan Heibeck1, Stephen M. Techtmann3, Terry C. Hazen4,5, Regina Lamendella1,2 1Department of Biology, Juniata College, 2Wright Labs, LLC, 3Department of Biological Sciences, Michigan Technological University, 4Biosciences Division, Oak Ridge National Laboratory, 5Department of Civil and Environmental Engineering, University of Tennessee Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.