Fisk University 1 article published in JoVE Biochemistry Expression, Purification, and Antimicrobial Activity of S100A12 Emmanuel Jackson1, Saffron Little1, Dana S. Franklin1, Jennifer A. Gaddy2,3, Steven M. Damo1,4 1Department of Life and Physical Sciences, Fisk University, 2Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs, 3Department of Medicine - Division of Infectious Diseases, Vanderbilt University Medical School, 4Department of Biochemistry, Vanderbilt University Here, we present a method to express and purify S100A12 (calgranulin C). We describe a protocol to measure its antimicrobial activity against the human pathogen H. pylori.