Institut de Recherches Cliniques de Montréal View Institution's Website 4 articles published in JoVE Medicine In utero Measurement of Heart Rate in Mouse by Noninvasive M-mode Echocardiography WooJin Kim1, Nabil G. Seidah1, Annik Prat1 1Laboratory of Biochemical Neuroendocrinology, Institut de Recherches Cliniques de Montréal Ultra-high frequency ultrasound is a powerful live imaging tool to examine cardiac abnormalities in small animals. Its noninvasiveness allows maintaining the physiologic state of embryos. Herein, we show the use of M-mode ultrasound to measure the heart rates of embryos at E18.5 in utero. Biology Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos Félix Legendre*1,2, Neal Cody*1, Carole Iampietro1, Julie Bergalet1, Fabio Alexis Lefebvre1,2, Gaël Moquin-Beaudry1,2, Olivia Zhang1, Xiaofeng Wang1, Eric Lécuyer1,2 1Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Biochemistry, Université de Montréal Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster. Medicine Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining Estelle Rousselet1, Jasna Kriz2, Nabil G. Seidah1 1Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 2CHUQ Research Centre, Laval University The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers. Neuroscience Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord Sébastien D. Langlois*1,2, Steves Morin*1, Patricia T. Yam1,3,4, Frédéric Charron1,2,5,6,7 1Molecular Biology of Neural Development, Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering, McGill University, 3Program in Neuroengineering, McGill University, 4Montreal Neurological Institute, 5Department of Anatomy and Cell Biology, McGill University, 6Department of Biology, McGill University, 7Department of Medicine, Universite de Montreal - University of Montreal This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.