Tecnologico de Monterrey 5 articles published in JoVE Biology In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye Francesca Lazzeri-Barcelo1, Pierpaolo Ciardo1, Barbara Leibiger1, Ingo B. Leibiger1, Per-Olof Berggren*1,2,3, Noah Moruzzi*1 1The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, 2Tecnológico de Monterrey, 3Diabetes Research Institute, Miller School of Medicine, University of Miami Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy. Biology Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy Perla Pérez-Treviño1, Bianca Nieblas1,2, Selma Romina López-Vaquera1, Noemí García1,2,3 1Experimental Medicine and Advanced Therapies, The Institute for Obesity Research, Tecnologico de Monterrey, 2Escuela de Medicina y Ciencias de la Salud, Tecnologico de Monterrey, 3Preclinical Research Unit, Tecnologico de Monterrey Here, we present a protocol to analyze changes in mitochondrial density and longitudinal distribution by live-skeletal muscle imaging using confocal microscopy for mitochondrial network scanning. Neuroscience Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research Lena Sophie Koch1, David Choy Buentello2,3, Kerensa Broersen1 1Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, 2Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, 3Department of Genetics, Harvard Medical School The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis. Neuroscience Three-Dimensional Motor Nerve Organoid Generation Tatsuya Osaki1,2, Siu Yu A. Chow1,2, Yui Nakanishi1,2, Joel Hernández1,3, Jiro Kawada4, Teruo Fujii1, Yoshiho Ikeuchi1,2 1Institute of Industrial Science, The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 3Faculty of Science and Engineering, Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc. This protocol provides a comprehensive procedure to fabricate human iPS cell-derived motor nerve organoid through spontaneous assembly of a robust bundle of axons extended from a spheroid in a tissue culture chip. Engineering Fabrication of 3D Carbon Microelectromechanical Systems (C-MEMS) Bidhan Pramanick1, Sergio O. Martinez-Chapa1, Marc Madou1,2, Hyundoo Hwang1,3 1School of Engineering and Sciences, Tecnologico de Monterrey, 2Department of Mechanical and Aerospace Engineering, University of California, 3BBB Inc Long and hollow glassy carbon microfibers were fabricated based on the pyrolysis of a natural product, human hair. The two fabrication steps of carbon microelectromechanical and carbon nanoelectromechanical systems, or C-MEMS and C-NEMS, are: (i) photolithography of a carbon-rich polymer precursor and (ii) pyrolysis of the patterned polymer precursor.
