Nemours/Alfred I. duPont Hospital for Children View Institution's Website 2 articles published in JoVE Chemistry Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol Xuyi Yue1,2, Rahul M. Nikam1,2, Heidi H. Kecskemethy1,2, Vinay V. R. Kandula2, Stephen J. Falchek3, Lauren W. Averill1,2, Sigrid A. Langhans1,4 1Diagnostic & Research PET/MRI Center, Nemours/Alfred I. duPont Hospital for Children, 2Department of Radiology, Nemours/Alfred I. duPont Hospital for Children, 3Department of Neurology, Nemours/Alfred I. duPont Hospital for Children, 4Department of Biomedical Research, Nemours/Alfred I. duPont Hospital for Children Here, we describe the radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-tryptophan, a positron emission tomography imaging agent for studying tryptophan metabolism, using a one-pot, two-step strategy in a radiochemistry synthesis system with good radiochemical yields, high enantiomeric excess, and high reliability. Biology Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons Chia-Yen Wu1, Dosh Whye1, Robert W. Mason1, Wenlan Wang1 1Nemours Biomedical Research, Alfred I. duPont Hospital for Children We developed a new protocol to improve efficiency of in vitro differentiation of mouse embryonic stem cells into motor neurons. The differentiated ES cells acquired motor neurons features as evidenced by expression of neuronal and motor neuron markers using immunohistochemical techniques.