Doane University 1 article published in JoVE Bioengineering Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System Arin L. Sutlief*1, Helena Valquier-Flynn1, Christina Wilson1, Marco Perez1, Hunter Kleinschmidt2, Brett J. Schofield2, Elizabeth Delmain3, Andrea E. Holmes1, Christopher D. Wentworth*4 1Department of Chemistry, Doane University, 2Department of Biology, Doane University, 3Department of Pathology and Microbiology, University of Nebraska Medical Center, 4Department of Physics and Engineering, Doane University Here, we describe the use of a higher-throughput microfluidic bioreactor coupled with a fluorescent microscope for the analysis of shear stress effects on Pseudomonas aeruginosa biofilms expressing green fluorescent proteins, including instrument set up, the determination of biofilm coverage, growth rate, and morphological properties.