University of Nebraska at Omaha 2 articles published in JoVE Medicine Utilizing Percutaneous Ventricular Assist Devices in Acute Myocardial Infarction Complicated by Cardiogenic Shock Shuktika Nandkeolyar1, Poonam Velagapudi2, Mir B. Basir3, Aditya S. Bharadwaj1 1Division of Cardiology, Loma Linda University Medical Center, 2Division of Cardiology, University of Nebraska, 3Division of Cardiology, Henry Ford Health System Percutaneous ventricular assist devices are increasingly being utilized in patients with acute myocardial infarction and cardiogenic shock. Herein, we discuss the mechanism of action and hemodynamic effects of such devices. We also review algorithms and best practices for the implantation, management and weaning of these complex devices. Immunology and Infection Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection Anna H. F. Andersen1,2, Stine S. F. Nielsen1,2, Rikke Olesen1,2, Katharina Mack1, Frederik Dagnæs-Hansen3, Niels Uldbjerg1,4, Lars Østergaard1,2, Ole S. Søgaard1,2, Paul W. Denton1,2,5, Martin Tolstrup1,2 1Department of Clinical Medicine, Aarhus University, 2Department of Infectious Diseases, Aarhus University Hospital, 3Department of Biomedicine, Aarhus University, 4Department of Gynecology and Obstetrics, Aarhus University Hospital, 5Department of Biology, University of Nebraska at Omaha We have developed a protocol for the generation and evaluation of a humanized and human immunodeficiency virus-infected NOG mouse model based on stem cell transplant, intravaginal human immunodeficiency virus exposure, and droplet digital PCR RNA quantification.